Sample Identification
Compound
Retatrutide (LY3437943; GIP/GLP-1/Glucagon Receptor Agonist)
Client
Numira Peptides · numira.cc
CAS Number
2381089-83-2
Date Received
2025-05-01
Molecular Formula
C₂₁₃H₃‴₅N‴₇O₆‴
Date Tested
2025-05-04
Molecular Weight
4766.45 g/mol
Date Reported
2025-05-10
Lot Number
NML-RET-250504
Test Methods
HPLC-UV · ICP-MS · Kinetic LAL
Appearance
White lyophilised powder
PubChem CID
2715879
HPLC-UV Purity & Content Analysis
Fig. 1 — HPLC-UV Chromatogram (214 nm) · Run: NML_RET_250504_1.d
mAU
0
250
500
750
1000
0
2
4
6
8
10
12
Time [min]
3.44 min
Retatrutide
9.28 min
11.15 min
APPEARANCE
#
Retention Time (min)
Area
% Area
Assignment
1
3.44
2.26E+02
0.63
Unknown impurity
2
9.28
3.54E+04
98.70
Retatrutide
3
11.15
2.40E+02
0.67
Unknown impurity
Bacterial Endotoxin Test (USP <85>)
Method
Result
Specification
Verdict
Kinetic LAL (USP <85>)
< 0.125 EU/mL
≤ 0.125 EU/mL
PASS
Elemental Impurities (USP <233> / ICH Q3D)
Element
Method
Result (ppm)
Limit (ppm)
Verdict
Arsenic (As)
ICP-MS
< 0.001
2.1
PASS
Cadmium (Cd)
ICP-MS
< 0.001
0.5
PASS
Lead (Pb)
ICP-MS
< 0.001
0.5
PASS
Mercury (Hg)
ICP-MS
< 0.001
3.0
PASS
Note: Peptide samples may contain counter-ions (TFA, acetate) and excipients (mannitol, sucrose) used as lyoprotectants and pH buffers. These are not detected by UV and are not classified as impurities under USP <233>.
Analysis Performed by
Dr. M. Chen, Ph.D. (Analytical Chemistry)
Senior Analytical Chemist · Nexus Molecular Labs
info@nexusmolecularlabs.com
2025-05-10
