Certificate of Analysis – NAD+ (1000mg)

Sample Identification

Compound	NAD+ (1000mg)	Client	Numira Peptides · numira.cc
CAS Number	53-84-9	Date Received	2026-06-17
Molecular Formula	C21H27N7O14P2	Date Tested	2026-06-21
Molecular Weight	663.43 g/mol	PubChem CID	5893
Lot Number	NML-NADP-260608	Test Methods	HPLC-UV · ICP-MS · Kinetic LAL
Appearance	White lyophilised powder	Date Reported	2026-06-25

HPLC-UV Purity & Content Analysis

1000mg

Ordered Amount

992mg

Measured Amount

99.18%

Purity (HPLC-UV)

99.2%

Recovery

Fig. 1 — HPLC-UV Chromatogram (214 nm) · Run: NML_NADPLUS_NADP-260608_1.d

APPEARANCE

#	Retention Time (min)	Area	% Area	Assignment
1	1.55	1.1E+02	0.30	Unknown impurity
2	3.18	4.010E+04	99.18	NAD+
3	5.02	1.1E+02	0.26	Unknown impurity

Bacterial Endotoxin Test (USP <85>)

Method	Result	Specification	Verdict
Kinetic LAL (USP <85>)	< 0.125 EU/mL	≤ 0.125 EU/mL	PASS

Elemental Impurities (USP <233> / ICH Q3D)

Element	Method	Result (ppm)	Limit (ppm)	Verdict
Arsenic (As)	ICP-MS	< 0.001	2.1	PASS
Cadmium (Cd)	ICP-MS	< 0.001	0.5	PASS
Lead (Pb)	ICP-MS	< 0.001	0.5	PASS
Mercury (Hg)	ICP-MS	< 0.001	3.0	PASS

Note: Peptide samples may contain counter-ions (TFA, acetate) and excipients (mannitol, sucrose) used as lyoprotectants and pH buffers. These are not detected by UV and are not classified as impurities under USP <233>.

Analysis Performed by
Dr. M. Chen, Ph.D. (Analytical Chemistry)
Senior Analytical Chemist · Nexus Molecular Labs
info@nexusmolecularlabs.com
2026-06-25