Certificate of Analysis – Melanotan I (10mg)

Sample Identification

Compound	Melanotan I (10mg)	Client	Numira Peptides · numira.cc
CAS Number	75921-69-6	Date Received	2026-06-16
Molecular Formula	C78H111N21O19	Date Tested	2026-06-20
Molecular Weight	1646.9 g/mol	PubChem CID	16132342
Lot Number	NML-MT1-260524	Test Methods	HPLC-UV · ICP-MS · Kinetic LAL
Appearance	White lyophilised powder	Date Reported	2026-06-24

HPLC-UV Purity & Content Analysis

10mg

Ordered Amount

9.90mg

Measured Amount

98.85%

Purity (HPLC-UV)

98.9%

Recovery

Fig. 1 — HPLC-UV Chromatogram (214 nm) · Run: NML_MELANOTAN1_MT1-260524_1.d

APPEARANCE

#	Retention Time (min)	Area	% Area	Assignment
1	2.68	1.2E+02	0.54	Unknown impurity
2	5.51	4.010E+04	98.85	Melanotan I
3	8.35	1.1E+02	0.46	Unknown impurity

Bacterial Endotoxin Test (USP <85>)

Method	Result	Specification	Verdict
Kinetic LAL (USP <85>)	< 0.125 EU/mL	≤ 0.125 EU/mL	PASS

Elemental Impurities (USP <233> / ICH Q3D)

Element	Method	Result (ppm)	Limit (ppm)	Verdict
Arsenic (As)	ICP-MS	< 0.001	2.1	PASS
Cadmium (Cd)	ICP-MS	< 0.001	0.5	PASS
Lead (Pb)	ICP-MS	< 0.001	0.5	PASS
Mercury (Hg)	ICP-MS	< 0.001	3.0	PASS

Note: Peptide samples may contain counter-ions (TFA, acetate) and excipients (mannitol, sucrose) used as lyoprotectants and pH buffers. These are not detected by UV and are not classified as impurities under USP <233>.

Analysis Performed by
Dr. M. Chen, Ph.D. (Analytical Chemistry)
Senior Analytical Chemist · Nexus Molecular Labs
info@nexusmolecularlabs.com
2026-06-24