Sample Identification
Compound
LL-37 (Human Cathelicidin Antimicrobial Peptide)
Client
Numira Peptides · numira.cc
CAS Number
154947-66-7
Date Received
2025-04-30
Molecular Formula
C₂₀₅H₃₄₀N₆₀O₅₃S
Date Tested
2025-05-03
Molecular Weight
4493.31 g/mol
Date Reported
2025-05-09
Lot Number
NML-LL37-250503
Test Methods
HPLC-UV · ICP-MS · Kinetic LAL
Appearance
White lyophilised powder
PubChem CID
16133605
HPLC-UV Purity & Content Analysis
Fig. 1 — HPLC-UV Chromatogram (214 nm) · Run: NML_LL37_250503_1.d
mAU
0
300
600
900
1200
0
2
4
6
8
10
12
Time [min]
2.85 min
LL-37
8.12 min
10.43 min
APPEARANCE
#
Retention Time (min)
Area
% Area
Assignment
1
2.85
1.88E+02
0.52
Unknown impurity
2
8.12
3.56E+04
98.80
LL-37
3
10.43
2.44E+02
0.68
Unknown impurity
Bacterial Endotoxin Test (USP <85>)
Method
Result
Specification
Verdict
Kinetic LAL (USP <85>)
< 0.125 EU/mL
≤ 0.125 EU/mL
PASS
Elemental Impurities (USP <233> / ICH Q3D)
Element
Method
Result (ppm)
Limit (ppm)
Verdict
Arsenic (As)
ICP-MS
< 0.001
2.1
PASS
Cadmium (Cd)
ICP-MS
< 0.001
0.5
PASS
Lead (Pb)
ICP-MS
< 0.001
0.5
PASS
Mercury (Hg)
ICP-MS
< 0.001
3.0
PASS
Note: Peptide samples may contain counter-ions (TFA, acetate) and excipients (mannitol, sucrose) used as lyoprotectants and pH buffers. These are not detected by UV and are not classified as impurities under USP <233>.
Analysis Performed by
Dr. M. Chen, Ph.D. (Analytical Chemistry)
Senior Analytical Chemist · Nexus Molecular Labs
info@nexusmolecularlabs.com
2025-05-09
