Sample Identification
Compound
GHK-Cu (Copper Peptide; Glycyl-L-histidyl-L-lysine copper(II))
Client
Numira Peptides · numira.cc
CAS Number
89030-95-5
Date Received
2025-05-03
Molecular Formula
C₁₄H₂₄CuN₆O₄
Date Tested
2025-05-06
Molecular Weight
403.90 g/mol
Date Reported
2025-05-12
Lot Number
NML-GHK-250506
Test Methods
HPLC-UV · ICP-MS · Kinetic LAL
Appearance
Blue-green lyophilised powder
PubChem CID
73758
HPLC-UV Purity & Content Analysis
Fig. 1 — HPLC-UV Chromatogram (214 nm) · Run: NML_GHK_250506_1.d
mAU
0
200
400
600
800
0
2
4
6
8
10
12
Time [min]
1.45 min
GHK-Cu
2.10 min
3.85 min
# Retention Time (min) Area % Area Assignment
1 1.45 2.95E+02 0.82 Unknown impurity 2 2.10 3.55E+04 98.50 GHK-Cu 3 3.85 2.45E+02 0.68 Unknown impurity
Bacterial Endotoxin Test (USP <85>)
Method Result Specification Verdict Kinetic LAL (USP <85>) < 0.125 EU/mL ≤ 0.125 EU/mL PASS
Elemental Impurities (USP <233> / ICH Q3D)
Element Method Result (ppm) Limit (ppm) Verdict
Arsenic (As) ICP-MS < 0.001 2.1 PASS Cadmium (Cd) ICP-MS < 0.001 0.5 PASS Lead (Pb) ICP-MS < 0.001 0.5 PASS Mercury (Hg) ICP-MS < 0.001 3.0 PASS Copper (Cu) — declared ICP-MS 1.56 N/A CONFORMS
Note: GHK-Cu contains copper as an integral component of the complex. Copper content is declared and confirmed by ICP-MS. Samples may also contain counter-ions (TFA, acetate) not detected by UV.
Analysis Performed by
Dr. M. Chen, Ph.D. (Analytical Chemistry)
Senior Analytical Chemist · Nexus Molecular Labs
info@nexusmolecularlabs.com
2025-05-12