Sample Identification
Compound
BPC-157 (Body Protection Compound-157)
Client
Numira Peptides · numira.cc
CAS Number
137525-51-0
Date Received
2025-04-28
Molecular Formula
C₆₂H₉₈N₁₆O₂₂
Date Tested
2025-05-01
Molecular Weight
1419.53 g/mol
Date Reported
2025-05-07
Lot Number
NML-BPC-250501
Test Methods
HPLC-UV · ICP-MS · Kinetic LAL
Appearance
White lyophilised powder
PubChem CID
9908089
HPLC-UV Purity & Content Analysis
Fig. 1 — HPLC-UV Chromatogram (214 nm) · Run: NML_BPC157_250501_1.d
mAU
0
300
600
900
1200
0
2
4
6
8
10
12
Time [min]
Time [min]
2.14 min
4.62 min
BPC-157
5.82 min
8.37 min
APPEARANCE
#
Retention Time (min)
Area
% Area
Assignment
1
2.14
1.23E+02
0.28
Unknown impurity
2
4.62
1.87E+02
0.43
Unknown impurity
3
5.82
4.31E+04
99.05
BPC-157
4
8.37
1.06E+02
0.24
Unknown impurity
Bacterial Endotoxin Test (USP <85>)
Method
Result
Specification
Verdict
Kinetic LAL (USP <85>)
< 0.125 EU/mL
≤ 0.125 EU/mL
PASS
Elemental Impurities (USP <233> / ICH Q3D)
Element
Method
Result (ppm)
Limit (ppm)
Verdict
Arsenic (As)
ICP-MS
< 0.001
2.1
PASS
Cadmium (Cd)
ICP-MS
< 0.001
0.5
PASS
Lead (Pb)
ICP-MS
< 0.001
0.5
PASS
Mercury (Hg)
ICP-MS
< 0.001
3.0
PASS
Note: Peptide samples may contain counter-ions (TFA, acetate) and excipients (mannitol, sucrose) used as lyoprotectants and pH buffers. These are not detected by UV and are not classified as impurities under USP <233>.
Analysis Performed by
Dr. M. Chen, Ph.D. (Analytical Chemistry)
Senior Analytical Chemist · Nexus Molecular Labs
info@nexusmolecularlabs.com
2025-05-07
