Certificate of Analysis – AHK-Cu (100mg)

Sample Identification

Compound	AHK-Cu (100mg)	Client	Numira Peptides · numira.cc
CAS Number	857066-90-1	Date Received	2026-06-18
Composition	Multi-component peptide fraction	Date Tested	2026-06-22
Molecular Weight	740.3 g/mol	Test Methods	HPLC-UV · ICP-MS · Kinetic LAL
Lot Number	NML-AHKCU-260518	Test Methods	HPLC-UV · ICP-MS · Kinetic LAL
Appearance	Blue lyophilized powder	Date Reported	2026-06-26

HPLC-UV Purity & Content Analysis

100mg

Ordered Amount

98.9mg

Measured Amount

98.63%

Purity (HPLC-UV)

98.9%

Recovery

Fig. 1 — HPLC-UV Chromatogram (214 nm) · Run: NML_AHKCU_AHKCU-260518_1.d

APPEARANCE

#	Retention Time (min)	Area	% Area	Assignment
1	2.21	1.2E+02	0.52	Unknown impurity
2	4.48	4.010E+04	98.63	AHK-Cu
3	7.02	1.1E+02	0.35	Unknown impurity

Bacterial Endotoxin Test (USP <85>)

Method	Result	Specification	Verdict
Kinetic LAL (USP <85>)	< 0.125 EU/mL	≤ 0.125 EU/mL	PASS

Elemental Impurities (USP <233> / ICH Q3D)

Element	Method	Result (ppm)	Limit (ppm)	Verdict
Arsenic (As)	ICP-MS	< 0.001	2.1	PASS
Cadmium (Cd)	ICP-MS	< 0.001	0.5	PASS
Lead (Pb)	ICP-MS	< 0.001	0.5	PASS
Mercury (Hg)	ICP-MS	< 0.001	3.0	PASS

Note: Peptide samples may contain counter-ions (TFA, acetate) and excipients (mannitol, sucrose) used as lyoprotectants and pH buffers. These are not detected by UV and are not classified as impurities under USP <233>.

Analysis Performed by
Dr. M. Chen, Ph.D. (Analytical Chemistry)
Senior Analytical Chemist · Nexus Molecular Labs
info@nexusmolecularlabs.com
2026-06-26